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Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement ArchiMer
Geiger, Marie.
The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...
Tipo: Text Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf
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First report on azaspiracid and yessotoxin groups detection in French shellfish ArchiMer
Amzil, Zouher; Sibat, Manoella; Royer, Florence; Savar, Veronique.
The French Phytoplankton and Phycotoxins monitoring network (REPHY) recently found positive or dubious negative shellfish samples using lipophilic toxins mouse bioassay. These samples were analyzed by liquid chromatography (LC) in combination with mass spectrometry (MS) to detect the following toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). Over the 2006-2007 period, chemical analyses revealed various lipophilic toxin profiles according to shellfish sampling locations. In addition to OA and/or PTX-2 and their derivatives, several other compounds were found for the first time in France: (1) during the summer of 2006, AZA-1 and AZA-2 in Queen...
Tipo: Text Palavras-chave: LC MS/MS analyses; Shellfish; Yessotoxin group; Azaspiracid group; Lipophilic toxins.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4560.pdf
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Lipophilic Toxin Profile in Mytilus galloprovincialis from the North Atlantic Coast of Morocco: LC-MS/MS and Mouse Bioassay Analyses ArchiMer
Ben Haddouch, Asia; Amanhi, Rachid; Amzil, Zouher; Taleb, Hamib; Rovillon, Georges-augustin; Adly, Farida; Loutfi, Mohammed.
Forthe Moroccan Phycotoxins Monitoring that is part of theSafety of theCoastal Monitoring Network(RSSL),shellfish sampleswereharvestedfromdifferentlocations at NorthAtlantic of Morocco whereharmful algaeblooms were known to have occurred. Forallshellfishsamples found positive by themouse bioassay fordiarrheicshellfishpoisoning(DSP)toxins,liquid chromatography coupled to tandem massspectrometry(LC-MS-MS) in order to search thefollowinglipophilictoxins: okadaic acid (OA), dinophysistoxins(DTXs), pectenotoxins(PTXs), azaspiracids(AZAs), yessotoxins(YTXs), spirolides(SPXs) and gymnodimines (GYMs).Theresultsrevealeddifferentlipophilictoxinprofiles as a function of theshellfish sampling location. It has beennoticed that allthesamples contained OA and its...
Tipo: Text Palavras-chave: Diarrheictoxins; Lipophilic toxins; LC-MS/MS; Mouse test; Shellfish.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00372/48342/48495.pdf
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Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey ArchiMer
Garcia-portela, Maria; Reguera, Beatriz; Sibat, Manoella; Altenburger, Andreas; Rodriguez, Francisco; Hess, Philipp.
Photosynthetic species of the genus Dinophysis are obligate mixotrophs with temporary plastids (kleptoplastids) that are acquired from the ciliate Mesodinium rubrum, which feeds on cryptophytes of the Teleaulax-Plagioselmis-Geminigera clade. A metabolomic study of the three-species food chain Dinophysis-Mesodinium-Teleaulax was carried out using mass spectrometric analysis of extracts of batch-cultured cells of each level of that food chain. The main goal was to compare the metabolomic expression of Galician strains of Dinophysis acuminata and D. acuta that were subjected to different feeding regimes (well-fed and prey-limited) and feeding on two Mesodinium (Spanish and Danish) strains. Both Dinophysis species were able to grow while feeding on both...
Tipo: Text Palavras-chave: Dinophysis physiology; Lipophilic toxins; Non-targeted analysis; Metabolomics.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00439/55077/56508.pdf
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Surveillance des phycotoxines dans les coquillages ArchiMer
Nicolas, Marina; Belin, Catherine; Favre, Pauline; Rudloff, Laurence.
This paper presents the French national system for monitoring three groups of marine biotoxins regulated in shellfish, implemented firstly in marine production areas by the REPHY REPHYTOX network of IFREMER and secondly at distribution level through the network of laboratories accredited by the Directorate General for Food within the framework of official controls. The European regulations, the nature of the shellfish toxins, and analytical methods used are presented. The sampling procedures and strategy, as well as the results obtained by each of the two systems mentioned, are presented and discussed.
Tipo: Text Palavras-chave: Phycotoxines; Coquillages; Toxines lipophiles; ASP; PSP; Surveillance; Phycotoxins; Shellfish; Lipophilic toxins; ASP; PSP; Surveillance.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00393/50407/51123.pdf
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